Cytoplasmic Dynein Promotes HIV-1 Uncoating

Viruses

Paulina Pawlica²  Lionel Berthoux¹ 

[1] Laboratory of Retrovirology, Department of Medical Biology and BioMed Research Center, Université du Québec à Trois-Rivières, 3351 Boulevard des Forges, Trois-Rivières, QC G9A 5H7, Canada; E-Mail

关键词: HIV-1;    uncoating;    capsid;    microtubules;    dynein;   

DOI  :  10.3390/v6114195

来源: mdpi

【 摘 要 】

Retroviral capsid (CA) cores undergo uncoating during their retrograde transport (toward the nucleus), and/or after reaching the nuclear membrane. However, whether HIV-1 CA core uncoating is dependent upon its transport is not understood. There is some evidence that HIV-1 cores retrograde transport involves cytoplasmic dynein complexes translocating on microtubules. Here we investigate the role of dynein-dependent transport in HIV-1 uncoating. To interfere with dynein function, we depleted dynein heavy chain (DHC) using RNA interference, and we over-expressed p50/dynamitin. In immunofluorescence microscopy experiments, DHC depletion caused an accumulation of CA foci in HIV-1 infected cells. Using a biochemical assay to monitor HIV-1 CA core disassembly in infected cells, we observed an increase in amounts of intact (pelletable) CA cores upon DHC depletion or p50 over-expression. Results from these two complementary assays suggest that inhibiting dynein-mediated transport interferes with HIV-1 uncoating in infected cells, indicating the existence of a functional link between HIV-1 transport and uncoating.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.