期刊论文详细信息
International Journal of Molecular Sciences
Analysis of Human TAAR8 and Murine Taar8b Mediated Signaling Pathways and Expression Profile
Jessica Mühlhaus2  Juliane Dinter2  Daniela Nürnberg2  Maren Rehders5  Maren Depke1  Janine Golchert1  Georg Homuth1  Chun-Xia Yi3  Silke Morin3  Josef Köhrle4  Klaudia Brix5  Matthias Tschöp3  Gunnar Kleinau2  Heike Biebermann2 
[1] Interfaculty Institute for Genetics and Functional Genomics, University Medicine and Ernst-Moritz-Arndt-University Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17487 Greifswald, Germany; E-Mails:;Institut für Experimentelle Pädiatrische Endokrinologie, Charité-Universitätsmedizin, Campus Virchow-Klinikum, Augustenburger Platz 1, 13353 Berlin, Germany; E-Mails:;Helmholtz Zentrum München, German Research Center for Environmental Health, Institute for Diabetes and Obesity, Business Campus Garching, Parkring 13, 85748 Garching, Germany; E-Mails:;Institut für Experimentelle Endokrinologie, Charité-Universitätsmedizin Campus Virchow-Klinikum, Augustenburger Platz 1, 13353 Berlin, Germany; E-Mail:;School of Engineering and Science, Research Center MOLIFE—Molecular Life Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany; E-Mails:
关键词: trace amine-associated receptor;    TAAR8;    3-T1AM;    thyronamine;    signaling pathways;    basal activity;   
DOI  :  10.3390/ijms151120638
来源: mdpi
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【 摘 要 】

The thyroid hormone derivative 3-iodothyronamine (3-T1AM) exerts metabolic effects in vivo that contradict known effects of thyroid hormones. 3-T1AM acts as a trace amine-associated receptor 1 (TAAR1) agonist and activates Gs signaling in vitro. Interestingly, 3-T1AM-meditated in vivo effects persist in Taar1 knockout-mice indicating that further targets of 3-T1AM might exist. Here, we investigated another member of the TAAR family, the only scarcely studied mouse and human trace-amine-associated receptor 8 (Taar8b, TAAR8). By RT-qPCR and locked-nucleic-acid (LNA) in situ hybridization, Taar8b expression in different mouse tissues was analyzed. Functionally, we characterized TAAR8 and Taar8b with regard to cell surface expression and signaling via different G-protein-mediated pathways. Cell surface expression was verified by ELISA, and cAMP accumulation was quantified by AlphaScreen for detection of Gs and/or Gi/o signaling. Activation of G-proteins Gq/11 and G12/13 was analyzed by reporter gene assays. Expression analyses revealed at most marginal Taar8b expression and no gender differences for almost all analyzed tissues. In heart, LNA-in situ hybridization demonstrated the absence of Taar8b expression. We could not identify 3-T1AM as a ligand for TAAR8 and Taar8b, but both receptors were characterized by a basal Gi/o signaling activity, a so far unknown signaling pathway for TAARs.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.

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