Chemical Compositions, Chromatographic Fingerprints and Antioxidant Activities of Andrographis Herba

Molecules

Yang Zhao³  Chun-Pin Kao²  Kun-Chang Wu³  Chi-Ren Liao³  Yu-Ling Ho¹ 

[1] Department of Nursing, Hungkuang University, Taichung 43302, Taiwan;Department of Nursing, Hsin Sheng College of Medical Care and Management, Taoyuan 32544, Taiwan;Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 40402, Taiwan

关键词: Andrographis Herba;    quantitative determination;    chromatographic fingerprint;    antioxidant activities;    correlation analysis;   

DOI  :  10.3390/molecules191118332

来源: mdpi

【 摘 要 】

This paper describes the development of an HPLC-UV-MS method for quantitative determination of andrographolide and dehydroandrographolide in Andrographis Herba and establishment of its chromatographic fingerprint. The method was validated for linearity, limit of detection and quantification, inter- and intra-day precisions, repeatability, stability and recovery. All the validation results of quantitative determination and fingerprinting methods were satisfactory. The developed method was then applied to assay the contents of andrographolide and dehydroandrographolide and to acquire the fingerprints of all the collected Andrographis Herba samples. Furthermore, similarity analysis and principal component analysis were used to reveal the similarities and differences between the samples on the basis of the characteristic peaks. More importantly, the DPPH free radical-scavenging and ferric reducing capacities of the Andrographis Herba samples were assayed. By bivariate correlation analysis, we found that six compounds are positively correlated to DPPH free radical scavenging and ferric reducing capacities, and four compounds are negatively correlated to DPPH free radical scavenging and ferric reducing capacities.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.