Synthesis of a Cu2+-Selective Probe Derived from Rhodamine and Its Application in Cell Imaging

Sensors

Chunwei Yu¹  Yingying Wen¹ 

关键词: rhodamine derivative;    fluorescence enhancement;    fluorescence imaging;   

DOI  :  10.3390/s141121375

来源: mdpi

【 摘 要 】

A new fluorescent probe P based on rhodamine for Cu²⁺ was synthesized and characterized. The new probe P showed high selectivity to Cu²⁺ over other tested metal ions. With optimal conditions, the proposed probe P worked in a wide linear range of 1.0 × 10⁻⁶−1.0 × 10⁻⁵ M with a detection limit of 3.3 × 10⁻⁷ M Cu²⁺ in ethanol-water solution (9:1, v:v, 20 mM HEPES, pH 7.0). Furthermore, it has been used for imaging of Cu²⁺ in living cells with satisfying results.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.