Glycoside Hydrolase Activities in Cell Walls of Sclerenchyma Cells in the Inflorescence Stems of Arabidopsis thaliana Visualized in Situ

Plants

Alicja Banasiak¹  Farid M. Ibatullin³  Harry Brumer³  Ewa J. Mellerowicz² 

[1] Institute of Experimental Biology, University of Wroclaw, 50-328 Wroclaw, Poland; E-Mail:;Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umea Plant Science Centre, 90183 Umea, Sweden;Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 106 91 Stockholm, Sweden; E-Mails:

关键词: cell wall;    xylem;    wood;    sclerenchyma;    glycoside hydrolase activity;    in situ activity;   

DOI  :  10.3390/plants3040513

来源: mdpi

【 摘 要 】

Techniques for in situ localization of gene products provide indispensable information for understanding biological function. In the case of enzymes, biological function is directly related to activity, and therefore, knowledge of activity patterns is central to understanding the molecular controls of plant development. We have previously developed a novel type of fluorogenic substrate for revealing glycoside hydrolase activity in planta, based on resorufin β-glycosides Here, we explore a wider range of such substrates to visualize glycoside hydrolase activities in Arabidopsis inflorescence stems in real time, especially highlighting distinct distribution patterns of these activities in the secondary cell walls of sclerenchyma cells. The results demonstrate that β-1,4-glucosidase, β-1,4-glucanase and β-1,4-galactosidase activities accompany secondary wall deposition. In contrast, xyloglucanase activity follows a different pattern, with the highest signal observed in mature cells, concentrated in the middle lamella. These data further the understanding of the process of cell wall deposition and function in sclerenchymatic tissues of plants.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.