International Journal of Molecular Sciences | |
Peptide Array on Cellulose Support—A Screening Tool to Identify Peptides with Dipeptidyl-Peptidase IV Inhibitory Activity within the Sequence of α-Lactalbumin | |
Isabelle M. E. Lacroix2  Eunice C. Y. Li-Chan1  | |
[1] Faculty of Land and Food Systems, Food, Nutrition and Health Program, the University of British Columbia, 2205 East Mall, Vancouver, BC V6T 1Z4, Canada; E-Mail | |
关键词: peptide array; dipeptidyl-peptidase IV (DPP-IV) inhibitors; α-lactalbumin; peptide–DPP-IV interaction; | |
DOI : 10.3390/ijms151120846 | |
来源: mdpi | |
【 摘 要 】
The inhibition of the enzyme dipeptidyl-peptidase IV (DPP-IV) is an effective pharmacotherapeutic approach for the management of type 2 diabetes. Recent findings have suggested that dietary proteins, including bovine α-lactalbumin, could be precursors of peptides able to inhibit DPP-IV. However, information on the location of active peptide sequences within the proteins is far from being comprehensive. Moreover, the traditional approach to identify bioactive peptides from foods can be tedious and long. Therefore, the objective of this study was to use peptide arrays to screen α-lactalbumin-derived peptides for their interaction with DPP-IV. Deca-peptides spanning the entire α-lactalbumin sequence, with a frame shift of 1 amino acid between successive sequences, were synthesized on cellulose membranes using “SPOT” technology, and their binding to and inhibition of DPP-IV was studied. Among the 114 α-lactalbumin-derived decamers investigated, the peptides 60WCKDDQNPHS69 (α
【 授权许可】
CC BY
© 2014 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
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