Rapid Detection of Chloramphenicol Residues in Aquatic Products Using Colloidal Gold Immunochromatographic Assay

Sensors

Chennan Zhou³  Xueyin Zhang³  Xinxin Huang²  Xishan Guo³  Qiang Cai¹ 

[1] Yangtze Delta Region Institute of Tsinghua University, Jiaxing 310013, China; E-Mail:;Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200000, China; E-Mail:;College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; E-Mails:

关键词: chloramphenicol;    colloidal gold nanoparticles;    fishery drug residues;    immunochromatographic assay;   

DOI  :  10.3390/s141121872

来源: mdpi

【 摘 要 】

A colloidal gold immunochromatographic assay (GICA) was developed for rapid detection of chloramphenicol (CAP) residues in aquatic products. A nitrocellulose (NC) membrane was used as the carrier, and the polyclonal CAP antibody was used as the marker protein. The average diameter of as-prepared colloidal gold nanoparticles (AuNPs) was about 20 nm. The optimal pH value of colloidal gold solutions and the amount of the antibody of CAP were 8.0 and 7.2 μg/mL, respectively. The CAP antibody was immobilized onto the conjugate pad after purification. The CAP conjugate and goat anti-rabbit IgG (secondary antibody) were coated onto the NC membrane. Next, the non-specific sites were blocked with 1% bovine serum albumin. The minimum detectable concentration of CAP in standard solution is 0.5 ng/mL, with good reproducibility. For the real samples from crucian carps injected with a single-dose of CAP in the dorsal muscles, the minimum detectable concentration of CAP residues was 0.5 μg/kg. The chromatographic analysis time was less than 10 min, and the strip had a long storage lifetime of more than 90 days at different temperatures. The strips provide a means for rapid detection of CAP residues in aquatic products.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.