The Effect of Lycii Radicis Cortex Extract on Bone Formation in Vitro and in Vivo

Molecules

Eunkuk Park⁵  Hyun-Seok Jin⁵  Doo-Yeoun Cho¹  Jeonghyun Kim⁵  Mun-Chang Kim⁵  Chun Whan Choi⁶  Yilan Jin⁵  Ji-Won Lee³  Jin-Hyok Park²  Yoon-Sok Chung⁴  Dam Huh²  Seon-Yong Jeong⁵ 

[1]Department of Family Practice and Community Health, Ajou University School of Medicine, Suwon 443-721, Korea

[2] E-Mail:

[3]Dongwoodang Pharmacy Co., Ltd., Yeongchen 770-864, Korea

[4] E-Mail:

[5]Korea Food Research Institute, Seongnam 463-746, Korea

[6] E-Mail:

[7]Department of Endocrinology and Metabolism, Ajou University School of Medicine, Suwon 443-721, Korea

[8] E-Mail:

[9]Department of Medical Genetics, Ajou University School of Medicine, Suwon 443-721, Korea

[10] E-Mails:

[11]Natural Products Research Institute, Gyeonggi Institute of Science & Technology Promotion, Suwon 443-721, Korea

[12] E-Mail:

关键词: herbal medicine;    osteoporosis;    Lycii Radicis Cortex;    osteoblast;    ovariectomized mice;    bone mineral density;   

DOI  :  10.3390/molecules191219594

来源: mdpi

【 摘 要 】

Osteoporosis is a common skeletal disease caused by decreased bone mass; it enhances the risk of bone fracture. This study aimed to discover novel herbal extract(s) for the treatment of osteoporosis. We screened 64 ethanol extracts of edible plants native to Korea for their ability to increase the cellular proliferation and differentiation of two osteoblastic cell lines: C3H10T1/2 and MC3T3-E1. We selected a Lycii Radicis Cortex (LRC), Lycium Chinese root bark as the primary candidate. Treatment with LRC extract showed enhanced alkaline phosphatase activity and increased expression of bone metabolic markers Alpl, Runx2, and Bglap genes in both osteoblastic cell lines. There was no effect on the osteoclastic differentiation of primary-cultured monocytes from the mouse bone marrows. Furthermore, the study examined the effect of LRC extract in vivo in ovariectomizd (OVX) mice for 8 weeks and 16 weeks, respectively. Bone mineral density (BMD) was significantly higher in LRC extract-administered group than in the non-LRC-administered OVX control group. The results indicated that LRC extract prevented the OVX-induced BMD loss in mice via promoting the differentiation of osteoblast linage cells. These results suggest that LRC extract may be a good natural herbal medicine candidate for the treatment of osteoporosis.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.