The Daidzein Metabolite, 6,7,4'-Trihydroxyisoflavone, Is a Novel Inhibitor of PKCα in Suppressing Solar UV-Induced Matrix Metalloproteinase 1

International Journal of Molecular Sciences

Tae-Gyu Lim¹  Jong-Eun Kim¹  Sung-Young Lee³  Jun Seong Park²  Myung Hun Yeom²  Hanyong Chen³  Ann M. Bode³  Zigang Dong³  Ki Won Lee¹ 

[1] World Class University Biomodulation Major, Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul 151-742, Korea; E-Mails:;Skin Research Institute, Amorepacific Corporation R&D Center, Yongin 446-829, Korea; E-Mails:;The Hormel Institute, University of Minnesota, Austin, MN 55912, USA; E-Mails:

关键词: matrix metalloproteinase 1;    protein kinase C (PKC)α;    photoaging;    6;    7;    4'-trihydroxyisoflavone;   

DOI  :  10.3390/ijms151121419

来源: mdpi

【 摘 要 】

Soy isoflavone is an attractive source of functional cosmetic materials with anti-wrinkle, whitening and skin hydration effects. After consumption, the majority of soy isoflavones are converted to their metabolites in the human gastrointestinal tract. To understand the physiological impact of soy isoflavone on the human body, it is necessary to evaluate and address the biological function of its metabolites. In this study, we investigated the effect of 6,7,4'-trihydroxyisoflavone (6,7,4'-THIF), a major metabolite of daidzein, against solar UV (sUV)-induced matrix metalloproteinases (MMPs) in normal human dermal fibroblasts. MMPs play a critical role in the degradation of collagen in skin, thereby accelerating the aging process of skin. The mitogen-activated protein/extracellular signal-regulated kinase (MEK)/extracellular signal-regulated kinase (ERK), mitogen-activated protein kinase (MKK)3/6/p38 and MKK4/c-Jun N-terminal kinases (JNK) signaling pathways are known to modulate MMP-1 function, and their activation by sUV was significantly reduced by 6,7,4'-THIF pretreatment. Our results also indicated that the enzyme activity of protein kinase C (PKC)α, an upstream regulator of MKKs signaling, is suppressed by 6,7,4'-THIF using the in vitro kinase assay. Furthermore, the direct interaction between 6,7,4'-THIF and endogenous PKCα was confirmed using the pull-down assay. Not only sUV-induced MMP-1 expression, but also sUV-induced signaling pathway activation were decreased in PKCα knockdown cells. Overall, we elucidated the inhibitory effect of 6,7,4'-THIF on sUV-induced MMPs and suggest PKCα as its direct molecular target.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.