A Fumonisins Immunosensor Based on Polyanilino-Carbon Nanotubes Doped with Palladium Telluride Quantum Dots

Sensors

Milua Masikini¹  Stephen N. Mailu¹  Abebaw Tsegaye¹  Njagi Njomo¹  Kerileng M. Molapo¹  Chinwe O. Ikpo¹  Christopher Edozie Sunday¹  Candice Rassie¹  Lindsay Wilson¹  Priscilla G. L. Baker¹ 

[1] SensorLab, Department of Chemistry, University of the Western Cape, Robert Sobukwe Road, Bellville 7535, Cape Town, South Africa;

关键词: anti-fumonisins antibody;    impedimetric immunosensor;    palladium telluride quantum dots;    poly(2;    5-dimethoxyaniline)-multi-wall carbon nanotubes;    electrochemical impedance spectroscopy;   

DOI  :  10.3390/s150100529

来源: mdpi

【 摘 要 】

An impedimetric immunosensor for fumonisins was developed based on poly(2,5-dimethoxyaniline)-multi-wall carbon nanotubes doped with palladium telluride quantum dots onto a glassy carbon surface. The composite was assembled by a layer-by-layer method to form a multilayer film of quantum dots (QDs) and poly(2,5-dimethoxyaniline)-multi-wall carbon nanotubes (PDMA-MWCNT). Preparation of the electrochemical immunosensor for fumonisins involved drop-coating of fumonisins antibody onto the composite modified glassy carbon electrode. The electrochemical impedance spectroscopy response of the FB₁ immunosensor (GCE/PT-PDMA-MWCNT/anti-Fms-BSA) gave a linear range of 7 to 49 ng L⁻¹ and the corresponding sensitivity and detection limits were 0.0162 kΩ L ng⁻¹ and 0.46 pg L⁻¹, respectively, hence the limit of detection of the GCE/PT-PDMA-MWCNT immunosensor for fumonisins in corn certified material was calculated to be 0.014 and 0.011 ppm for FB₁, and FB₂ and FB₃, respectively. These results are lower than those obtained by ELISA, a provisional maximum tolerable daily intake (PMTDI) for fumonisins (the sum of FB₁, FB₂, and FB₃) established by the Joint FAO/WHO expert committee on food additives and contaminants of 2 μg kg⁻¹ and the maximum level recommended by the U.S. Food and Drug Administration (FDA) for protection of human consumption (2–4 mg L⁻¹).

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.