Activation of the Ubiquitin Proteasome Pathway by Silk Fibroin Modified Chitosan Nanoparticles in Hepatic Cancer Cells

International Journal of Molecular Sciences

Ming-Hui Yang⁴  Tze-Wen Chung¹  Yi-Shan Lu⁶  Yi-Ling Chen³  Wan-Chi Tsai⁸  Shiang-Bin Jong⁶  Shyng-Shiou Yuan⁴  Pao-Chi Liao⁵  Po-Chiao Lin⁷  Yu-Chang Tyan² 

[1] Department of Biomedical Engineering, National Yang-Ming University, Taipei 112, Taiwan; E-Mail:;Translational Research Center, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan;Department of Nuclear Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan; E-Mail:;Department of Medical Research, KaohsiungMedical University Hospital, Kaohsiung 807, Taiwan; E-Mails:;Department of Environmental and Occupational Health, National Cheng Kung University, Tainan 701, Taiwan; E-Mail:;Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan; E-Mails:;Department of Chemistry, National Sun Yat-sen University, Kaohsiung 804, Taiwan; E-Mail:;Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan; E-Mail:

关键词: silk fibroin;    chitosan nanoparticle;    biomaterial;    proteomics;    ubiquitin proteasome pathway;   

DOI  :  10.3390/ijms16011657

来源: mdpi

【 摘 要 】

Silk fibroin (SF) is a protein with bulky hydrophobic domains and can be easily purified as sericin-free silk-based biomaterial. Silk fibroin modified chitosan nanoparticle (SF-CSNP), a biocompatible material, has been widely used as a potential drug delivery system. Our current investigation studied the bio-effects of the SF-CSNP uptake by liver cells. In this experiment, the characterizations of SF-CSNPs were measured by particle size analysis and protein assay. The average size of the SF-CSNP was 311.9 ± 10.7 nm, and the average zeta potential was +13.33 ± 0.3 mV. The SF coating on the SF-CSNP was 6.27 ± 0.17 μg/mL. Moreover, using proteomic approaches, several proteins involved in the ubiquitin proteasome pathway were identified by analysis of differential protein expressions of HepG2 cell uptake the SF-CSNP. Our experimental results have demonstrated that the SF-CSNP may be involved in liver cancer cell survival and proliferation.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.