2-DE Mapping of the Blue Mussel Gill Proteome: The Usual Suspects Revisited

Proteomes

Bບtrice Rocher³  Florence Bultelle³  Philippe Chan²  Frank Le Foll³  Julie Letendre³  Tiphaine Monsinjon³  Stéphanie Olivier³  Romain Pn³  Agnès Poret³  David Vaudry¹  Thomas Knigge³ 

[1]Neuronal and Neuroendocrine Differentiation and Communication (DC2N), Inserm U982, University of Rouen, 76821 Mont-Saint-Aignan, France

[2] E-Mail:

[3]PISSARO Proteomic Platform, Institute for Research and Innovation in Biomedicine, University of Rouen, 76821 Mont-Saint-Aignan, France

[4] E-Mail:

[5]Laboratory of Ecotoxicology—Aquatic Environments, UMR I-02 SEBIO, Le Havre University, 76063 Le Havre, France

[6] E-Mails:

关键词: 2-DE;    mass spectrometry;    proteoforms;    post-translational modifications;    bivalve mollusc;    environmental stress;    ecotoxicology;   

DOI  :  10.3390/proteomes3010003

来源: mdpi

【 摘 要 】

The Blue Mussel (Mytilus edulis, L. 1758) is an ecologically important and commercially relevant bivalve. Because of its ability to bioconcentrate xenobiotics, it is also a widespread sentinel species for environmental pollution, which has been used in ecotoxicological studies for biomarker assessment. Consequently, numerous proteomics studies have been carried out in various research contexts using mussels of the genus Mytilus, which intended to improve our understanding of complex physiological processes related to reproduction, adaptation to physical stressors or shell formation and for biomarker discovery. Differential-display 2-DE proteomics relies on an extensive knowledge of the proteome with as many proteoforms identified as possible. To this end, extensive characterization of proteins was performed in order to increase our knowledge of the Mytilus gill proteome. On average, 700 spots were detected on 2-DE gels by colloidal blue staining, of which 122 different, non-redundant proteins comprising 203 proteoforms could be identified by tandem mass spectrometry. These proteins could be attributed to four major categories: (i) “metabolism”, including antioxidant defence and degradation of xenobiotics; (ii) “genetic information processing”, comprising transcription and translation as well as folding, sorting, repair and degradation; (iii) “cellular processes”, such as cell motility, transport and catabolism; (iv) “environmental information processing”, including signal transduction and signalling molecules and interaction. The role of cytoskeleton proteins, energetic metabolism, chaperones/stress proteins, protein trafficking and the proteasome are discussed in the light of the exigencies of the intertidal environment, leading to an enhanced stress response, as well as the structural and physiological particularities of the bivalve gill tissue.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.