Mini-P-gp and P-gp Co-Expression in Brown Trout Erythrocytes: A Prospective Blood Biomarker of Aquatic Pollution

Diagnostics

Emeline Valton¹  Christian Amblard⁴  François Desmolles²  Bruno Combourieu⁵  Frédérique Penault-Llorca³  Mahchid Bamdad¹ 

[1] ERTICa—EA 4677, Département Génie Biologique, Institut Universitaire de Technologie, Clermont Université—Université d’Auvergne, Ensemble universitaire des Cézeaux, B.P. 86-63172 Aubiere, France; E-Mail:;Fédération pour la Pêche et la Protection du Milieu Aquatique du Puy de Dôme (F.P.P.M.A. 63), 14 Allée des Eaux et Forêts, Site de Marmilhat Sud, 63370 Lempdes, France; E-Mail:;ERTICa—EA 4677, Centre Jean Perrin, Clermont Université—Université d’Auvergne, 58 rue Montalembert, B.P. 392-63011 Clermont-Ferrand, France; E-Mail:;LMGE—UMR CNRS 6023, Clermont Université—Université Blaise Pascal, B.P. 80026-63171 Aubiere, France; E-Mail:;Rovaltain Research Company, 1 avenue de la gare, B.P. 10313, 26958 Valence Cedex 09, France; E-Mail:

关键词: blood biomarker;    erythrocytes;    brown trout;    mini-P-gp;    P-gp;    river pollution;   

DOI  :  10.3390/diagnostics5010010

来源: mdpi

【 摘 要 】

In aquatic organisms, such as fish, blood is continually exposed to aquatic contaminants. Multidrug Resistance (MDR) proteins are ubiquitous detoxification membrane pumps, which recognize various xenobiotics. Moreover, their expression is induced by a large class of drugs and pollutants. We have highlighted the co-expression of a mini P-gp of 75 kDa and a P-gp of 140 kDa in the primary culture of brown trout erythrocytes and in the erythrocytes of wild brown trout collected from three rivers in the Auvergne region of France. In vitro experiments showed that benzo[a]pyrene, a highly toxic pollutant model, induced the co-expression of mini-P-gp and P-gp in trout erythrocytes in a dose-dependent manner and relay type response. Similarly, in the erythrocytes of wild brown trout collected from rivers contaminated by a mixture of PAH and other multi-residues of pesticides, mini-P-gp and P-gp were able to modulate their expression, according to the nature of the pollutants. The differential and complementary responses of mini-P-gp and P-gp in trout erythrocytes suggest the existence in blood cells of a real protective network against xenobiotics/drugs. This property could be exploited to develop a blood biomarker of river pollution.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.