The Cluster [Re6Se8I6]3− Induces Low Hemolysis of Human Erythrocytes in Vitro: Protective Effect of Albumin

International Journal of Molecular Sciences

Edgardo Rojas-Mancilla¹  Alexis Oyarce⁴  Viviana Verdugo⁴  Zhiping Zheng³  Rodrigo Ramírez-Tagle² 

[1] Universidad Bernardo O’ Higgins, Departamento de Ciencias Químicas y Biológicas, General Gana 1780, Santiago 8370854, Chile; E-Mail:;Universidad Bernardo O’ Higgins, Laboratorio de Bionanotecnología, General Gana 1780, Santiago 8370854, Chile;Department of Chemistry, the University of Arizona, Tucson, AZ 85721, USA; E-Mail:;Universidad Bernardo O’Higgins, Escuela de Tecnología Médica, General Gana 1780, Santiago 8370854, Chile; E-Mails:

关键词: cluster;    hemolysis;    erythrocytes;    albumin;   

DOI  :  10.3390/ijms16011728

来源: mdpi

【 摘 要 】

The cluster Re₆Se₈I₆³⁻ has been shown to induce preferential cell death of a hepatic carcinoma cell line, thus becoming a promising anti-cancer drug. Whether this cluster induces acute hemolysis or if it interacts with albumin remains unclear. The effect of acute exposure of human red blood cells to different concentrations of the cluster with and without albumin is described. Red blood cells from healthy donors were isolated, diluted at 1% hematocrit and exposed to the cluster (25–150 µM) at 37 °C, under agitation. Hemolysis and morphology were analyzed at 1 and 24 h. The potential protection of 0.1% albumin was also evaluated. Exposition to therapeutic doses of the cluster did not induce acute hemolysis. Similar results were observed following 24 h of exposition, and albumin slightly reduced hemolysis levels. Furthermore, the cluster induced alteration in the morphology of red blood cells, and this was prevented by albumin. Together, these results indicate that the cluster Re₆Se₈I₆³⁻ is not a hemolytic component and induces moderate morphological alterations of red blood cells at high doses, which are prevented by co-incubation with albumin. In conclusion, the cluster Re₆Se₈I₆³⁻ could be intravenously administered in animals at therapeutic doses for in vivo studies.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.