Fluorescent Cellular Assay for Screening Agents Inhibiting Pseudomonas aeruginosa Adherence

Sensors

Libuᘞ Nosková²  Bo៮na Kubíčková²  Lucie Vašková²  Barbora Bláhová²  Michaela Wimmerová¹  Marie Stiborová² 

[1] Central European Institute of Technology, Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic; E-Mail:;Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 8, 128 40 Prague 2, Czech Republic; E-Mails:

关键词: cystic fibrosis;    lungs;    bacterial infection;    lectin;    chicken antibody;    cell line;    dual fluorescence;   

DOI  :  10.3390/s150101945

来源: mdpi

【 摘 要 】

Antibodies against Pseudomonas aeruginosa (PA) lectin, PAIIL, which is a virulence factor mediating the bacteria binding to epithelium cells, were prepared in chickens and purified from egg yolks. To examine these antibodies as a prophylactic agent preventing the adhesion of PA we developed a well plate assay based on fluorescently labeled bacteria and immortalized epithelium cell lines derived from normal and cystic fibrosis (CF) human lungs. The antibodies significantly inhibited bacteria adhesion (up to 50%) in both cell lines. In agreement with in vivo data, our plate assay showed higher susceptibility of CF cells towards the PA adhesion as compared to normal epithelium. This finding proved the reliability of the developed experimental system.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.