Cross-Amplification of Vicia sativa subsp. sativa Microsatellites across 22 Other Vicia Species

Molecules

Sebastin Raveendar³  Gi-An Lee³  Young-Ah Jeon³  Yun Jeong Lee³  Jung-Ro Lee³  Gyu-Taek Cho³  Joon-Hyeong Cho²  Jong-Hyun Park¹  Kyung-Ho Ma³  Jong-Wook Chung³ 

[1] Food Grain Policy Division, Ministry of Agriculture, Food and Rural Affairs, Sejong-si 339-012, Korea;Department of Biological and Environmental Science, Dongguk University, Seoul 100-175, Korea;National Agrobiodiversity Centre, National Academy of Agricultural Science, Rural Development Administration, Jeonju 560-500, Korea

关键词: cDNA-SSR;    genetic diversity;    transferability;    Vicia L.;   

DOI  :  10.3390/molecules20011543

来源: mdpi

【 摘 要 】

The temperate and herbaceous genus Vicia L. is a member of the legume tribe Fabeae of the subfamily Papilionoideae. The genus Vicia comprises 166 annual or perennial species distributed mainly in Europe, Asia, and North America, but also extending to the temperate regions of South America and tropical Africa. The use of simple sequence repeat (SSR) markers for Vicia species has not been investigated as extensively as for other crop species. In this study, we assessed the potential for cross-species amplification of cDNA microsatellite markers developed from common vetch (Vicia sativa subsp. sativa). For cross-species amplification of the SSRs, amplification was carried out with genomic DNA isolated from two to eight accessions of 22 different Vicia species. For individual species or subspecies, the transferability rates ranged from 33% for V. ervilia to 82% for V. sativa subsp. nigra with an average rate of 52.0%. Because the rate of successful SSR marker amplification generally correlates with genetic distance, these SSR markers are potentially useful for analyzing genetic relationships between or within Vicia species.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.