Low Toxicity of Deoxynivalenol-3-Glucoside in Microbial Cells

Toxins

Tadahiro Suzuki²  Yumiko Iwahashi¹ 

[1] Applied Microbiology Division, National Food Research Institute, 2-1-12 Kannon-dai, Tsukuba, Ibaraki 305-8642, Japan; E-Mail

关键词: deoxynivalenol-3-glucoside;    DNA microarray;    yeast;    Chlamydomonas reinhardtii;   

DOI  :  10.3390/toxins7010187

来源: mdpi

【 摘 要 】

Host plants excrete a glucosylation enzyme onto the plant surface that changes mycotoxins derived from fungal secondary metabolites to glucosylated products. Deoxynivalenol-3-glucoside (DON3G) is synthesized by grain uridine diphosphate-glucosyltransferase, and is found worldwide, although information on its toxicity is lacking. Here, we conducted growth tests and DNA microarray analysis to elucidate the characteristics of DON3G. The Saccharomyces cerevisiae PDR5 mutant strain exposed to DON3G demonstrated similar growth to the dimethyl sulfoxide control, and DNA microarray analysis revealed limited differences. Only 10 genes were extracted, and the expression profile of stress response genes was similar to that of DON, in contrast to metabolism genes like SER3, which encodes 3-phosphoglycerate dehydrogenase. Growth tests with Chlamydomonas reinhardtii also showed a similar growth rate to the control sample. These results suggest that DON3G has extremely low toxicity to these cells, and the glucosylation of mycotoxins is a useful protective mechanism not only for host plants, but also for other species.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.