Tyrosine Sulfation as a Protein Post-Translational Modification

Molecules

Yuh-Shyong Yang²  Chen-Chu Wang²  Bo-Han Chen²  You-Hua Hou²  Kuo-Sheng Hung¹  Yi-Chih Mao² 

[1] Department of Neurosurgery, Center of Excellence for Clinical Trial and Research, Taipei Medical University-Wan Fang Medical Center, Taipei 11696, Taiwan;Department of Biological Science and Technology, National Chiao Tung University, 75 Po-Ai Street, Hsinchu 30068, Taiwan; E-Mails:

关键词: sulfate;    organic sulfate;    post-translational modification (PTM);    protein tyrosine sulfation (PTS);    tyrosylprotein sulfotransferase (TPST);    3'-phosphoadenosine 5'-phosphosulfate (PAPS);   

DOI  :  10.3390/molecules20022138

来源: mdpi

【 摘 要 】

Integration of inorganic sulfate into biological molecules plays an important role in biological systems and is directly involved in the instigation of diseases. Protein tyrosine sulfation (PTS) is a common post-translational modification that was first reported in the literature fifty years ago. However, the significance of PTS under physiological conditions and its link to diseases have just begun to be appreciated in recent years. PTS is catalyzed by tyrosylprotein sulfotransferase (TPST) through transfer of an activated sulfate from 3'-phosphoadenosine-5'-phosphosulfate to tyrosine in a variety of proteins and peptides. Currently, only a small fraction of sulfated proteins is known and the understanding of the biological sulfation mechanisms is still in progress. In this review, we give an introductory and selective brief review of PTS and then summarize the basic biochemical information including the activity and the preparation of TPST, methods for the determination of PTS, and kinetics and reaction mechanism of TPST. This information is fundamental for the further exploration of the function of PTS that induces protein-protein interactions and the subsequent biochemical and physiological reactions.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.