Development and Characterization of Polymorphic Microsatellite Markers (SSRs) for an Endemic Plant, Pseudolarix amabilis (Nelson) Rehd. (Pinaceae)

Molecules

Qi-Fang Geng³  Jun Liu³  Lin Sun³  Hong Liu¹  Yan Ou-Yang³  Ying Cai³  Xin-Sheng Tang⁴  Hong-Wei Zhang²  Zhong-Sheng Wang³  Shu-Qing An³ 

[1] International Center for Tropical Botany, Department of Earth and Environment, Florida International University, Miami, FL 33199, USA; E-Mail:;Administration of Zhejiang Qingliangfeng National Nature Reserve, Lin’an 311321, China; E-Mail:;School of Life Science, Nanjing University, Nanjing 210093, China; E-Mails:;College of Life and Environment Sciences, Huangshan University, Huangshan 245041, China; E-Mail:

关键词: microsatellite markers;    ESTs;    SSRs;    Pseudolarix amabilis;    Pinaceae;   

DOI  :  10.3390/molecules20022685

来源: mdpi

【 摘 要 】

Pseudolarix (Pinaceae) is a vulnerable (sensu IUCN) monotypic genus restricted to southeastern China. To better understand levels of genetic diversity, population structure and gene flow among populations of P. amabilis, we developed five compound SSR markers and ten novel polymorphic expressed sequence tags (EST) derived microsatellites. The results showed that all 15 loci were polymorphic with the number of alleles per locus ranging from two to seven. The expected and observed heterozygosities varied from 0.169 to 0.752, and 0.000 to 1.000, respectively. The inbreeding coefficient ranged from −0.833 to 1.000. These markers will contribute to research on genetic diversity and population genetic structure of P. amabilis, which in turn will contribute to the species conservation.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.