Association of OCT-Derived Drusen Measurements with AMD-Associated Genotypic SNPs in the Amish Population

Journal of Clinical Medicine

Venkata Ramana Murthy Chavali⁴  Bruno Diniz¹  Jiayan Huang²  Gui-Shuang Ying²  SriniVas R. Sadda¹  Dwight Stambolian⁴  Lindsay Farrer³ 

[1] Doheny Eye Institute, Los Angeles, CA 90033, USA; E-Mails:;Center for Preventive Ophthalmology and Biostatistics, Department of Ophthalmology, University of Pennsylvania, Philadelphia, PA 19104, USA; E-Mails:;Department of Ophthalmology, University of Pennsylvania, 313B Stellar-Chance Labs, 422 Curie Blvd., Philadelphia, PA 19104, USA; E-Mail;Department of Ophthalmology, University of Pennsylvania, 313B Stellar-Chance Labs, 422 Curie Blvd., Philadelphia, PA 19104, USA; E-Mail:

关键词: age-related macular degeneration;    AMD;    Older Order Amish;    CFH;    SYN3;    OCT;    drusen area;    drusen volume;    RPE atrophy;    Cirrus HD-OCT;   

DOI  :  10.3390/jcm4020304

来源: mdpi

【 摘 要 】

Purpose: To investigate the association of optical coherence tomography (OCT)-derived drusen measures in Amish age-related macular degeneration (AMD) patients with known loci for macular degeneration. Methods: Members of the Old Order Amish community in Pennsylvania ages 50 and older were assessed for drusen area, volume and regions of retinal pigment epithelium (RPE) atrophy using a Cirrus High-Definition OCT. Measurements were obtained in the macula region within a central circle (CC) of 3 mm in diameter and a surrounding perifoveal ring (PR) of 3 to 5 mm in diameter using the Cirrus OCT RPE analysis software. Other demographic information, including age, gender and smoking status, were collected. Study subjects were further genotyped to determine their risk for the AMD-associated SNPs in the SYN3, LIPC, ARMS2, C3, CFB, CETP, CFI and CFH genes using TaqMan genotyping assays. The association of genotypes with OCT measures were assessed using linear trend p-values calculated from univariate and multivariate generalized linear models. Results: 432 eyes were included in the analysis. Multivariate analysis (adjusted by age, gender and smoking status) confirmed the known significant association between AMD and macular drusen with the number of CFH risk alleles for the drusen area (the area increased 0.12 mm² for a risk allele increase, p < 0.01), drusen volume (the volume increased 0.01 mm³ for a risk allele increase, p ≤ 0.05) and the area of RPE atrophy (the area increased 0.43 mm² for a risk allele increase, p = 0.003). SYN3 risk allele G is significantly associated with larger area PR (the area increased 0.09 mm² for a risk allele increase, p = 0.03) and larger drusen volume in the central circle (the volume increased 0.01 mm³ for a risk allele increase, p = 0.04). Conclusion: Among the genotyped SNPs tested, the CFH risk genotype appears to play a major role in determining the drusen phenotype in the Amish AMD population.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.