The Synthetic Antimicrobial Peptide Pexiganan and Its Nanoparticles (PNPs) Exhibit the Anti-Helicobacter pylori Activity in Vitro and in Vivo

Molecules

Xiao-Lin Zhang¹  An-Min Jiang²  Zhong-You Ma¹  Xian-Bao Li¹  You-Yi Xiong¹  Jin-Feng Dou¹  Jian-Fei Wang¹ 

[1]The Department of Pharmacy, Food and Drug School, Anhui Science and Technology University, Fengyang 233100, China

[2] E-Mails:

[3]The School of Life Science, University of Science and Technology of China, Hefei 230032, China

[4] E-Mail:

关键词: antimicrobial peptide;    pexiganan;    nanoparticles;    Helicobacter pylori;   

DOI  :  10.3390/molecules20033972

来源: mdpi

【 摘 要 】

The aim of this study was to probe the potential anti-H. pylori activity of the synthetic antimicrobial peptide pexiganan, which is an analog of the peptide magainin, and its nanoparticles (PNPs) that were prepared in our laboratory. To compare their antibacterial effects in vitro and in vivo, studies of H. pylori growth inhibition, kinetics and resistance assays were undertaken. The gastric mucoadhesive efficiency and H. pylori clearance efficiency of pexiganan and PNPs were evaluated in rats and mice infected with H. pylori. The eradication of H. pylori was determined using urease tests and a microbial culture method. We observed that PNPs adhered to gastric mucosa more effectively owing to a prolonged stay in the stomach, which resulted in a more effective H. pylori clearance. In addition, PNPs had greater anti-H. pylori effect than pexiganan in infected mice. The amount of pexiganan required to eradicate H. pylori was significantly less using PNPs than the corresponding pexiganan suspension. The results confirmed that PNPs improved peptide stability in the stomach and more effectively eradicated H. pylori from mice stomachs than pexiganan.

【 授权许可】

CC BY   
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