Engineering a Ca++-Sensitive (Bio)Sensor from the Pore-Module of a Potassium Channel

Sensors

Mattia Lorenzo DiFrancesco²  Sabrina Gazzarrini²  Cristina Arrigoni²  Giulia Romani¹  Gerhard Thiel³  Anna Moroni² 

[1] Institute of Biophysics-Milan section, National Research Council, Via Celoria 26, Milano 20133, Italy; E-Mail:;Department of Biosciences, University of Milan, Via Celoria 26, Milano 20133, Italy; E-Mails:;Department of Biology, Technische Universität Darmstadt, Schnittspahnstrasse 3, Darmstadt 64287, Germany; E-Mail:

关键词: biosensor;    Kcv;    calcium;    calmodulin;    synthetic ion channel;   

DOI  :  10.3390/s150304913

来源: mdpi

【 摘 要 】

Signals recorded at the cell membrane are meaningful indicators of the physiological vs. pathological state of a cell and will become useful diagnostic elements in nanomedicine. In this project we present a coherent strategy for the design and fabrication of a bio-nano-sensor that monitors changes in intracellular cell calcium concentration and allows an easy read out by converting the calcium signal into an electrical current in the range of microampere that can be easily measured by conventional cell electrophysiology apparatus.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.