Untargeted NMR-Based Methodology in the Study of Fruit Metabolites

Molecules

Anatoly Petrovich Sobolev⁴  Luisa Mannina⁴  Noemi Proietti⁴  Simone Carradori²  Maria Daglia¹  Anna Maria Giusti³  Riccarda Antiochia⁵  Donatella Capitani⁴ 

[1]Department of Drug Sciences, Medicinal Chemistry and Pharmaceutical Technology Section, Pavia University, Via Taramelli 12, 27100 Pavia, Italy

[2] E-Mail:

[3]Department of Pharmacy, “G. D’Annunzio” University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti Scalo (CH), Italy

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[5]Department of Experimental Medicine, Sapienza University of Rome, Piazzale Aldo Moro 5, I-00185 Rome, Italy

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[7]Magnetic Resonance Laboratory “Annalaura Segre”, Institute of Chemical Methodologies, National Research Council (CNR), I-00015 Monterotondo (RM), Italy

[8] E-Mails:

[9]Department of Drug Chemistry and Technologies, Sapienza University of Rome, Piazzale Aldo Moro 5, I-00185 Rome, Italy

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关键词: NMR;    fruits;    metabolomics;    primary metabolites;    secondary metabolites;   

DOI  :  10.3390/molecules20034088

来源: mdpi

【 摘 要 】

In this review, fundamental aspects of the untargeted NMR-based methodology applied to fruit characterization are described. The strategy to perform the structure elucidation of fruit metabolites is discussed with some examples of spectral assignments by 2D experiments. Primary ubiquitous metabolites as well as secondary species-specific metabolites, identified in different fruits using an untargeted ¹H-NMR approach, are summarized in a comprehensive way. Crucial aspects regarding the quantitative elaboration of spectral data are also discussed. The usefulness of the NMR-based metabolic profiling was highlighted using some results regarding quality, adulteration, varieties and geographical origin of fruits and fruit-derived products such as juices.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.