Profiling of Measles-Specific Humoral Immunity in Individuals Following Two Doses of MMR Vaccine Using Proteome Microarrays

Viruses

Iana H. Haralambieva²  Whitney L. Simon²  Richard B. Kennedy²  Inna G. Ovsyannikova²  Nathaniel D. Warner¹  Diane E. Grill¹  Gregory A. Poland² 

[1] Division of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, MN 55905, USA; E-Mails:;Mayo Vaccine Research Group, Mayo Clinic, Guggenheim 611C, 200 First Street SW, Rochester, MN 55905, USA; E-Mails:

关键词: measles;    measles-mumps-rubella vaccine;    measles virus;    viral vaccines;    antibodies;    neutralizing;    immunity;    humoral;    protein array analysis;   

DOI  :  10.3390/v7031113

来源: mdpi

【 摘 要 】

Introduction: Comprehensive evaluation of measles-specific humoral immunity after vaccination is important for determining new and/or additional correlates of vaccine immunogenicity and efficacy. Methods: We used a novel proteome microarray technology and statistical modeling to identify factors and models associated with measles-specific functional protective immunity in 150 measles vaccine recipients representing the extremes of neutralizing antibody response after two vaccine doses. Results: Our findings demonstrate a high seroprevalence of antibodies directed to the measles virus (MV) phosphoprotein (P), nucleoprotein (N), as well as antibodies to the large polymerase (L) protein (fragment 1234 to 1900 AA). Antibodies to these proteins, in addition to anti-F antibodies (and, to a lesser extent, anti-H antibodies), were correlated with neutralizing antibody titer and/or were associated with and predictive of neutralizing antibody response. Conclusion: Our results identify antibodies to specific measles virus proteins and statistical models for monitoring and assessment of measles-specific functional protective immunity in vaccinated individuals.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.