Three-Dimensional Cell Culture: A Breakthrough in Vivo

International Journal of Molecular Sciences

Delphine Antoni¹  Hélène Burckel²  Elodie Josset²  Georges Noel¹ 

[1] Radiotherapy Department, Paul Strauss Cancer Center, 3, rue de la Porte de l’Hôpital, 67065 Strasbourg Cedex, France; E-Mail:;Radiobiology Laboratory, EA 3430, Strasbourg University, Paul Strauss Cancer Center, 3, rue de la Porte de l’Hôpital, 67065 Strasbourg Cedex, France; E-Mails:

关键词: cell culture;    three-dimensional cell culture;    in vivo;   

DOI  :  10.3390/ijms16035517

来源: mdpi

【 摘 要 】

Cell culture is an important tool for biological research. Two-dimensional cell culture has been used for some time now, but growing cells in flat layers on plastic surfaces does not accurately model the in vivo state. As compared to the two-dimensional case, the three-dimensional (3D) cell culture allows biological cells to grow or interact with their surroundings in all three dimensions thanks to an artificial environment. Cells grown in a 3D model have proven to be more physiologically relevant and showed improvements in several studies of biological mechanisms like: cell number monitoring, viability, morphology, proliferation, differentiation, response to stimuli, migration and invasion of tumor cells into surrounding tissues, angiogenesis stimulation and immune system evasion, drug metabolism, gene expression and protein synthesis, general cell function and in vivo relevance. 3D culture models succeed thanks to technological advances, including materials science, cell biology and bioreactor design.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.