Microarray Analysis on Gene Regulation by Estrogen, Progesterone and Tamoxifen in Human Endometrial Stromal Cells

International Journal of Molecular Sciences

Chun-E Ren¹  Xueqiong Zhu⁷  Jinping Li⁸  Christian Lyle⁵  Sean Dowdy²  Karl C. Podratz²  David Byck⁶  Hai-Bin Chen³  Shi-Wen Jiang⁸  Robert J. Norman⁴ 

[1] Department of Obstetrics and Gynecology, Center of Reproductive Medicine, Affiliated Hospital of Weifang Medical University, Weifang 261043, China; E-Mail:;Department of Obstetrics and Gynecology, Mayo Clinic, Rochester, MN 55905, USA; E-Mails:;Department of Histology and Embryology, Shantou University Medical College, Shantou 515041, China;Department of Obstetrics and Gynecology, Center of Reproductive Medicine, Affiliated Hospital of Weifang Medical University, Weifang 261043, China; E-Mail;Department of Biology, Savannah State University, Savannah, GA 31419, USA; E-Mail:;Department of Obstetrics and Gynecology, Memorial Health University Medical Center, Savannah, GA 31404, USA; E-Mail:;Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325001, China; E-Mail:;Department of Biomedical Science, Mercer University School of Medicine, Savannah, GA 31404, USA; E-Mail:

关键词: stroma;    uterus;    estrogen;    progesterone;    tamoxifen;    transcription;   

DOI  :  10.3390/ijms16035864

来源: mdpi

【 摘 要 】

Epithelial stromal cells represent a major cellular component of human uterine endometrium that is subject to tight hormonal regulation. Through cell-cell contacts and/or paracrine mechanisms, stromal cells play a significant role in the malignant transformation of epithelial cells. We isolated stromal cells from normal human endometrium and investigated the morphological and transcriptional changes induced by estrogen, progesterone and tamoxifen. We demonstrated that stromal cells express appreciable levels of estrogen and progesterone receptors and undergo different morphological changes upon hormonal stimulation. Microarray analysis indicated that both estrogen and progesterone induced dramatic alterations in a variety of genes associated with cell structure, transcription, cell cycle, and signaling. However, divergent patterns of changes, and in some genes opposite effects, were observed for the two hormones. A large number of genes are identified as novel targets for hormonal regulation. These hormone-responsive genes may be involved in normal uterine function and the development of endometrial malignancies.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.