Evaluation of an Ultrafiltration-Based Procedure for Simultaneous Recovery of Diverse Microbes in Source Waters

Water

Amy M. Kahler³  Trisha B. Johnson²  Donghyun Hahn²  Jothikumar Narayanan³  Gordana Derado³  Vincent R. Hill³  Say-Leong Ong¹ 

[1] Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA;;Atlanta Research and Education Foundation, Decatur, GA 30033, USA; E-Mails:;Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA; E-Mails:

关键词: groundwater;    surface water;    ultrafiltration;    pathogen detection;    Centricon Plus-70;    polyethylene glycol;    immunomagnetic separation (IMS);   

DOI  :  10.3390/w7031202

来源: mdpi

【 摘 要 】

In this study, hollow-fiber ultrafiltration (UF) was assessed for recovery of Escherichia coli, Clostridium perfringens spores, Cryptosporidium parvum oocysts, echovirus 1, and bacteriophages MS2 and ΦX174 from ground and surface waters. Microbes were seeded into twenty-two 50-L water samples that were collected from the Southeastern United States and concentrated to ~500 mL by UF. Secondary concentration was performed for C. parvum by centrifugation followed by immunomagnetic separation. Secondary concentration for viruses was performed using centrifugal ultrafilters or polyethylene glycol precipitation. Nine water quality parameters were measured in each water sample to determine whether water quality data correlated with UF and secondary concentration recovery efficiencies. Average UF recovery efficiencies were 66%–95% for the six enteric microbes. Average recovery efficiencies for the secondary concentration methods were 35%–95% for C. parvum and the viruses. Overall, measured water quality parameters were not significantly associated with UF recovery efficiencies. However, recovery of ΦX174 was negatively correlated with turbidity. The recovery data demonstrate that UF can be an effective method for concentrating diverse microbes from ground and surface waters. This study highlights the utility of tangential-flow hollow fiber ultrafiltration for recovery of bacteria, viruses, and parasites from large volume environmental water samples.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.