Bending Elasticity Modulus of Giant Vesicles Composed of Aeropyrum Pernix K1 Archaeal Lipid

Life

Julia Genova¹  Nataᘚ Poklar Ulrih²  Veronika Kralj-Iglič⁴  Aleš Iglič³  Isak Bivas¹ 

[1] Institute of Solid State Physics, Bulgarian Academy of Sciences, 72, Tzarigradsko Chaussee Blvd., 1784 Sofia, Bulgaria; E-Mails:;Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia; E-Mail:;Laboratory of Biophysics, Faculty of Electrical Engineering, University of Ljubljana, Tržaška 25, SI-1000 Ljubljana, Slovenia; E-Mail:;Laboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, Zdravstvena 5, SI-1000 Ljubljana, Slovenia

关键词: giant vesicles;    Aeropyrum pernix K1;    archaeal lipids;    membrane bending elasticity;    membrane bending elasticity modulus;    phospholipid bilayer;    flickering;    cell shapes;   

DOI  :  10.3390/life5021101

来源: mdpi

【 摘 要 】

Thermally induced shape fluctuations were used to study elastic properties of giant vesicles composed of archaeal lipids C_25,25-archetidyl (glucosyl) inositol and C_25,25-archetidylinositol isolated from lyophilised Aeropyrum pernix K1 cells. Giant vesicles were created by electroformation in pure water environment. Stroboscopic illumination using a xenon flash lamp was implemented to remove the blur effect due to the finite integration time of the camera and to obtain an instant picture of the fluctuating vesicle shape. The mean weighted value of the bending elasticity modulus k_c of the archaeal membrane determined from the measurements meeting the entire set of qualification criteria was (1.89 ± 0.18) × 10⁻¹⁹ J, which is similar to the values obtained for a membrane composed of the eukaryotic phospholipids SOPC (1.88 ± 0.17) × 10⁻¹⁹ J and POPC (2.00 ± 0.21) × 10⁻¹⁹ J. We conclude that membranes composed of archaeal lipids isolated from Aeropyrum pernix K1 cells have similar elastic properties as membranes composed of eukaryotic lipids. This fact, together with the importance of the elastic properties for the normal circulation through blood system, provides further evidence in favor of expectations that archaeal lipids could be appropriate for the design of drug delivery systems.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.