Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria

Microorganisms

Tami L. McTaggart³  David A. C. Beck³  Usanisa Setboonsarng³  Nicole Shapiro¹  Tanja Woyke¹  Mary E. Lidstrom³  Marina G. Kalyuzhnaya²  Ludmila Chistoserdova³ 

[1] DOE Joint Genome Institute, Walnut Creek, CA 94598, USA; E-Mails:;Department of Microbiology, University of Washington, Seattle, WA 98195, USA; E-Mail:;Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA; E-Mails:

关键词: methylotrophy;    genomics;    Arthrobacter;    Bacillus;    Mycobacterium;    Lake Washington;   

DOI  :  10.3390/microorganisms3010094

来源: mdpi

【 摘 要 】

Gram-positive methylotrophic bacteria have been known for a long period of time, some serving as model organisms for characterizing the specific details of methylotrophy pathways/enzymes within this group. However, genome-based knowledge of methylotrophy within this group has been so far limited to a single species, Bacillus methanolicus (Firmicutes). The paucity of whole-genome data for Gram-positive methylotrophs limits our global understanding of methylotrophy within this group, including their roles in specific biogeochemical cycles, as well as their biotechnological potential. Here, we describe the isolation of seven novel strains of Gram-positive methylotrophs that include two strains of Bacillus and five representatives of Actinobacteria classified within two genera, Arthrobacter and Mycobacterium. We report whole-genome sequences for these isolates and present comparative analysis of the methylotrophy functional modules within these genomes. The genomic sequences of these seven novel organisms, all capable of growth on methylated amines, present an important reference dataset for understanding the genomic basis of methylotrophy in Gram-positive methylotrophic bacteria. This study is a major contribution to the field of methylotrophy, aimed at closing the gap in the genomic knowledge of methylotrophy within this diverse group of bacteria.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.