An Improved Electrochemical Aptasensor for Chloramphenicol Detection Based on Aptamer Incorporated Gelatine

Sensors

Ezat Hamidi-Asl²  Freddy Dardenne¹  Ronny Blust¹  Karolien De Wael² 

[1] Sphere Research Group, Department of Biology, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium; E-Mails:;AXES Research Group, Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium; E-Mail:

关键词: aptamers;    gelatine;    chloramphenicol;    electrochemical sensor;   

DOI  :  10.3390/s150407605

来源: mdpi

【 摘 要 】

Because of the biocompatible properties of gelatine and the good affinity of aptamers for their targets, the combination of aptamer and gelatine type B is reported as promising for the development of biosensing devices. Here, an aptamer for chloramphenicol (CAP) is mixed with different types of gelatine and dropped on the surface of disposable gold screen printed electrodes. The signal of the CAP reduction is investigated using differential pulse voltammetry. The diagnostic performance of the sensor is described and a detection limit of 1.83 × 10⁻¹⁰ M is found. The selectivity and the stability of the aptasensor are studied and compared to those of other CAP sensors described in literature.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.