International Journal of Environmental Research and Public Health | |
Isolation and Molecular Characterization of Free-Living Amoebae from Different Water Sources in Italy | |
Margherita Montalbano Di Filippo4  Maristella Santoro4  Piero Lovreglio3  Rosa Monno1  Carmen Capolongo1  Carla Calia1  Luciana Fumarola1  Rossella D𠆚lfonso2  Federica Berrilli4  David Di Cave4  Roberto Spurio5  Duarte Tito5  Letizia Brandi5  | |
[1] Department of Basic Medical Science, Neuroscience and Sense Organ, University of Bari, Piazza G. Cesare 11, 70124 Bari, Italy; E-Mails:;Department of Systems Medicine, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, Italy; E-Mail:;Interdisciplinary Department of Medicine, University of Bari, Piazza G. Cesare 11, 70124 Bari, Italy; E-Mail:;Department of Experimental Medicine and Surgery, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, Italy; E-Mails:Department of Experimental Medicine and Surgery, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, Italy; | |
关键词: free-living amoebae; molecular characterization; water sources; Italy; | |
DOI : 10.3390/ijerph120403417 | |
来源: mdpi | |
【 摘 要 】
Free-living amoebae (FLA) are protozoa ubiquitous in Nature, isolated from a variety of environments worldwide. In addition to their natural distribution, some species have been found to be pathogenic to humans. In the present study a survey was conducted in order to evaluate the presence and to characterize at molecular level the isolates of amoebic organisms collected from different water sources in Italy. A total of 160 water samples were analyzed by culture and microscopic examination. FLA were found in 46 (28.7%) of the investigated water samples. Groundwater, well waters, and ornamental fountain waters were the sources with higher prevalence rates (85.7%, 50.0%, and 45.9%, respectively). Identification of FLA species/genotypes, based on the 18S rDNA regions, allowed to identify 18 (39.1%)
【 授权许可】
CC BY
© 2015 by the authors; licensee MDPI, Basel, Switzerland.
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