Microarrays | |
3D Cell Culture in Alginate Hydrogels | |
Therese Andersen1  Pia Auk-Emblem2  Michael Dornish2  Mohammad Reza Lornejad-Schr2  | |
[1] FMC BioPolymer AS, Industriveien 33, 1337 Sandvika, Norway; | |
关键词: alginate; hydrogel; 3D; drug development; tissue regeneration; drug discovery; AlgiMatrix®; NovaMatrix®-3D; beads; bioprinting; | |
DOI : 10.3390/microarrays4020133 | |
来源: mdpi | |
【 摘 要 】
This review compiles information regarding the use of alginate, and in particular alginate hydrogels, in culturing cells in 3D. Knowledge of alginate chemical structure and functionality are shown to be important parameters in design of alginate-based matrices for cell culture. Gel elasticity as well as hydrogel stability can be impacted by the type of alginate used, its concentration, the choice of gelation technique (ionic or covalent), and divalent cation chosen as the gel inducing ion. The use of peptide-coupled alginate can control cell–matrix interactions. Gelation of alginate with concomitant immobilization of cells can take various forms. Droplets or beads have been utilized since the 1980s for immobilizing cells. Newer matrices such as macroporous scaffolds are now entering the 3D cell culture product market. Finally, delayed gelling, injectable, alginate systems show utility in the translation of
【 授权许可】
CC BY
© 2015 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202003190014657ZK.pdf | 1583KB | download |