Dried Pomegranate Potentiates Anti-Osteoporotic and Anti-Obesity Activities of Red Clover Dry Extracts in Ovariectomized Rats

Nutrients

Su Jin Kang³  Beom Rak Choi⁴  Seung Hee Kim⁴  Hae Yeon Yi⁴  Hye Rim Park⁴  Dong Chul Kim⁵  Seong Hun Choi¹  Chang Hyun Han²  Soo Jin Park³  Chang Hyun Song³  Sae Kwang Ku³ 

[1] Department of Histology and Anatomy, College of Korean Medicine, Daegu Haany University, 1, Hannydaero, Gyeongsan, Gyeongsangbuk-Do 712-715, Korea; E-Mail:;Department of Medical History & Literature Group, Korea Institute of Oriental Medicine, Daejeon, 305-811, Korea; E-Mail:;The Medical Research Center for Globalization of Herbal Medicine, Daegu Haany University, Gyeongsan, Gyeongsangbuk-Do 712-715, Korea; E-Mails:;Research Institute, Health-Love Co., Ltd., Anyang, 431-060, Korea; E-Mails:;Department of Korean Obstetrics & Gynecology, College of Korean Medicine, Daegu Haany University, Daegu, 704-123, Korea; E-Mail:

关键词: red clover extracts;    dried pomegranate concentrate powder;    2:1 mixture;    anti-climacteric effects;    ovariectomy;    rats;   

DOI  :  10.3390/nu7042622

来源: mdpi

【 摘 要 】

Red clover (RC) shows potential activity against menopausal symptoms and pomegranates have antioxidative and beneficial effects on postmenopausal symptoms; thus, we investigated whether the anti-climacteric activity of RC could be enhanced by the addition of dried pomegranate concentrate powder (PCP) extracts in ovariectomized (OVX) rats. Regarding the anti-osteoporotic effects, bone mineral density increased significantly in OVX induced rats treated with 60 and 120 mg/kg of an RC:PCP 2:1 mixture, respectively, compared with OVX control rats. Additionally, femoral, tibia, and L4 bone resorption was decreased in OVX induced control rats treated with the RC:PCP 2:1 mixture (60 and 120 mg/kg), respectively, compared with OVX control rats. Regarding anti-obesity effects, the OVX induced rats treated with 60 and 120 mg/kg of the RC:PCP 2:1 mixture showed a decrease in total fat pad thickness, the mean diameters of adipocytes and the body weights gain compared with OVX induced control rats. The estradiol and bone-specific alkaline phosphatase levels were significantly increased in OVX induced rats treated with the RC:PCP 2:1 mixture (120 mg/kg) compared with OVX induced control rats, also, the uterine atrophy was significantly inhibited in 60 and 120 mg/kg of the RC:PCP 2:1 mixture treatment compared with OVX control rats. In conclusion, our results indicate that PCP enhanced the anti-climacteric effects of RC in OVX rats. The RC:PCP 2:1 mixture used in this study may be a promising new potent and protective agent for relieving climacteric symptoms.

【 授权许可】

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