Synthesis, Photophysical Characterization, and Photoinduced Antibacterial Activity of Methylene Blue-loaded Amino- and Mannose-Targeted Mesoporous Silica Nanoparticles

Molecules

Oriol Planas¹  Roger Bresolí-Obach¹  Jaume Nos¹  Thibault Gallavardin²  Rubén Ruiz-González²  Montserrat Agut²  Santi Nonell¹ 

[1] Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Spain;

关键词: antimicrobial photodynamic therapy (aPDT);    singlet oxygen (¹O₂);    mesoporous silica nanoparticle (MSNP);    methylene blue;    drug delivery system;    mannose;    E. coli;    P. aeruginosa;   

DOI  :  10.3390/molecules20046284

来源: mdpi

【 摘 要 】

Over the last 20 years, the number of pathogenic multi-resistant microorganisms has grown steadily, which has stimulated the search for new strategies to combat antimicrobial resistance. Antimicrobial photodynamic therapy (aPDT), also called photodynamic inactivation, is emerging as a promising alternative to treatments based on conventional antibiotics. We have explored the effectiveness of methylene blue-loaded targeted mesoporous silica nanoparticles (MSNP) in the photodynamic inactivation of two Gram negative bacteria, namely Escherichia coli and Pseudomonas aeruginosa. For E. coli, nanoparticle association clearly reduced the dark toxicity of MB while preserving its photoinactivation activity. For P. aeruginosa, a remarkable difference was observed between amino- and mannose-decorated nanoparticles. The details of singlet oxygen production in the nanoparticles have been characterized, revealing the presence of two populations of this cytotoxic species. Strong quenching of singlet oxygen within the nanoparticles is observed.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.