Selection of Reference Genes for MicroRNA Quantitative Expression Analysis in Chinese Perch, Siniperca chuatsi

International Journal of Molecular Sciences

Xin Zhu²  Yu-Long Li²  Dun-Xue Chen²  Ping Wu²  Tan Yi²  Tao Chen¹  Jian-She Zhang²  Wu-Ying Chu² 

[1] College of Veterinary Medicine, Hunan Agriculture University, Changsha 410128, China; E-Mail:;Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China; E-Mails:

关键词: miRNA;    real-time PCR;    reference genes;    relative quantification;    Siniperca chuatsi;   

DOI  :  10.3390/ijms16048310

来源: mdpi

【 摘 要 】

Real-time quantitative reverse transcription PCR (RT-qPCR) is one of the most effective and sensitive techniques in gene expression assay, for which selection of reference genes is a prerequisite. In teleost species, such as Chinese perch, the expression profiling of miRNAs as reference genes for RT-qPCR has not been intensively studied. In the present study, the expression profiles of six miRNAs (miR-101a, miR-146a, miR-22a, miR-23a, miR-26a and let-7a) and one small nuclear RNA (U6) were assayed with RT-qPCR in different adult tissues, developmental stages and growth conditions of Chinese perch, Siniperca chuatsi. The analyses revealed that embryonic developmental stage is an important variability factor in the expression stability of miRNAs. All six miRNAs exhibited better expression consistency than U6 in most of the conditions examined, and therefore, they may be more suitable as a reference gene for miRNA quantification. When different tissues and developmental stages were considered, miR-22a demonstrated the most consistent expression pattern, and the best combination of reference genes was miR-22a and miR-23a. Our study offers useful data for selecting miRNAs as reference genes for RT-qPCR analysis of miRNAs in teleost fishes under different conditions.

【 授权许可】

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.