Molecular and Chemical Analysis of the Lipopolysaccharide from Aeromonas hydrophila Strain AH-1 (Serotype O11)

Marine Drugs

Susana Merino³  Rocío Canals²  Yuriy A. Knirel¹  Juan M. Tomás³ 

[1] N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow V-334, Russia; E-Mail:;Institute of Integrative Biology, University of Liverpool, Crown Street, L69 7ZB Liverpool, UK; E-Mail:;Department of Microbiology, Faculty of Biology, University of Barcelona, Diagonal 643, 08071 Barcelona, Spain; E-Mail:

关键词: Aeromonas;    genomics;    proteomics;    lipopolysaccharide;    O11-antigen;    LPS-core;   

DOI  :  10.3390/md13042233

来源: mdpi

【 摘 要 】

A group of virulent Aeromonas hydrophila, A. sobria, and A. veronii biovar sobria strains isolated from humans and fish have been described; these strains classified to serotype O11 are serologically related by their lipopolysaccharide (LPS) O-antigen (O-polysaccharide), and the presence of an S-layer consisting of multiple copies of a crystalline surface array protein with a molecular weight of 52 kDa in the form of a crystalline surface array which lies peripheral to the cell wall. A. hydrophila strain AH-1 is one of them. We isolated the LPS from this strain and determined the structure of the O-polysaccharide, which was similar to that previously described for another strain of serotype O11. The genetics of the O11-antigen showed the genes (wb_O11 cluster) in two sections separated by genes involved in biosynthesis and assembly of the S-layer. The O11-antigen LPS is an example of an ABC-2-transporter-dependent pathway for O-antigen heteropolysaccharide (disaccharide) assembly. The genes involved in the biosynthesis of the LPS core (waa_O11 cluster) were also identified in three different chromosome regions being nearly identical to the ones described for A. hydrophila AH-3 (serotype O34). The genetic data and preliminary chemical analysis indicated that the LPS core for strain AH-1 is identical to the one for strain AH-3.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.