Chromatography | |
The Evaluation of Magnetic Polymethacrylate-based Microspheres Used for Solid Phase DNA Micro-Extraction | |
Štěpánka Trachtová1  Hana Zapletalová3  Alena Španová1  Daniel Horák2  Hana Kolářová3  Bohuslav Rittich1  | |
[1] Faculty of Chemistry, Brno University of Technology, Purkyňova 118, CZ-612 00 Brno, Czech Republic;Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovský Sq. 2, CZ-162 06 Prague, Czech Republic;Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacký University Olomouc, Hněvotínská 3, CZ-775 15 Olomouc, Czech Republic | |
关键词: magnetic microspheres; quantitative polymerase chain reaction; inhibitory effect; atomic force microscopy; | |
DOI : 10.3390/chromatography2020156 | |
来源: mdpi | |
【 摘 要 】
Using magnetic particles as a solid-phase extraction system is the most frequently used micro-technique for DNA isolation. Particles with a complete covering of magnetic cores by a polymer are hence preferred. Quantitative polymerase chain reaction (qPCR) was used for the evaluation of the polymer coating efficiency of hydrophilic magnetic poly(2-hydroxyethyl methacrylate-
【 授权许可】
CC BY
© 2015 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
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RO202003190014236ZK.pdf | 1408KB | download |