Investigation of Förster Resonance Energy Transfer (FRET) and Competition of Fluorescent Dyes on DNA Microparticles

International Journal of Molecular Sciences

Jieun Kim²  Jae Sung Lee²  Jong Bum Lee¹ 

[1] Department of Chemical Engineering, University of Seoul, Seoul 130-743, Korea;

关键词: DNA technology;    DNA microparticles;    bioimaging;    Förster resonance energy transfer (FRET);   

DOI  :  10.3390/ijms16047738

来源: mdpi

【 摘 要 】

Fluorescent labeling is widely used to investigate the structural stability and changes to DNA nano- and microstructures. Despite this, the conventional method for observing DNA structures has several limitations in terms of cost-efficiency. This paper introduces a DNA spherical particle stained with DNA intercalating dyes (SYBR Green and SYTOX Orange) as tracers and reports the interaction between multiple dyes. The interference between the dyes was analyzed in terms of Förster resonance energy transfer (FRET) and competition. The changes in the fluorescence intensity by FRET were uniform, regardless of the sequence. The competition effect could occur when several dyes were added simultaneously. These properties are expected to help in the design of multicolor tracers in bioimaging and environmental applications.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.