An Ultra-High Performance Liquid Chromatographic-Tandem Mass Spectrometric Method for the Determination of Sinomenine in Human Plasma after Transdermal Delivery of the Zhengqing Fengtongning Injection

Molecules

Tingbo Chen²  Zheng Xiang²  Gengting Dong²  Pei Luo²  Ping Qiu³  Shenzhi Wang¹  Baoming Huang²  Yingyi Wen³  Feichi Wu³  Liang Liu²  Hua Zhou² 

[1] The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, China; E-Mail:;State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, China; E-Mails:;Hunan Zhengqing Pharmaceutical Group Limited, Huaihua 418005, China; E-Mails:

关键词: sinomenine;    Zhengqing Fengtongning Injection;    transdermal delivery;    human plasma;    concentration;    liquid chromatography-tandem mass spectrometry;   

DOI  :  10.3390/molecules20046454

来源: mdpi

【 摘 要 】

A sensitive, precise and selective ultra-high performance liquid chromatography method coupled with triple-quadrupole mass spectrometry was developed and validated for the determination of trace amounts of sinomenine (ng/mL) in minute volumes of human plasma. Fifty microliter plasma samples were precipitated using methanol to extract sinomenine. Separation was carried out on a C₁₈ column with a water and acetonitrile mobile phase gradient with formic acid as an additive. The mass spectrometry data were obtained in the positive ion mode, and the transition of multiple reactions was monitored at m/z 330.2→181.0 for sinomenine quantification. The working assay range for sinomenine was linear from 0.1173 to 15.02 ng/mL with the lower limit of quantification of 0.1173 ng/mL. The precision and accuracy of the method was less than 15% in intra-day and inter-day experiments with a matrix effect of less than 6.5%. After validation, the quantitative method was applied to analyze sinomenine levels in human plasma after transdermal delivery of the Zhengqing Fengtongning Injection. The results showed that some samples contained sinomenine within the concentration range 0.4131–4.407 ng/mL.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.