NAPPA as a Real New Method for Protein Microarray Generation

Microarrays

Paula Dໞz²  Mar໚ González-González²  Luc໚ Lourido³  Rosa M. Dégano¹  Nieves Ibarrola¹  Juan Casado-Vela⁴  Joshua LaBaer⁵  Manuel Fuentes² 

[1]Proteomics Unit, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca 37007, Spain

[2] E-Mails:

[3]Department of Medicine and General Cytometry Service-Nucleus, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca 37007, Spain

[4] E-Mails:

[5]Rheumatology Division, ProteoRed/ISCIII Proteomics Group, INIBIC, Hospital Universitario de A Coruña, A Coruña 15006, Spain

[6] E-Mail:

[7]Biotechnology National Centre, Spanish National Research Council (CSIC), Madrid 28049, Spain

[8] E-Mail:

[9]Biodesign Institute, Arizona State University, 1001 South McAllister Avenue, Tempe, AZ 85287, USA

[10] E-Mail:

关键词: protein microarray;    NAPPA;    high-throughput screening;    biomarker;    protein-protein interaction;    microarray generation;   

DOI  :  10.3390/microarrays4020214

来源: mdpi

【 摘 要 】

Nucleic Acid Programmable Protein Arrays (NAPPA) have emerged as a powerful and innovative technology for the screening of biomarkers and the study of protein-protein interactions, among others possible applications. The principal advantages are the high specificity and sensitivity that this platform offers. Moreover, compared to conventional protein microarrays, NAPPA technology avoids the necessity of protein purification, which is expensive and time-consuming, by substituting expression in situ with an in vitro transcription/translation kit. In summary, NAPPA arrays have been broadly employed in different studies improving knowledge about diseases and responses to treatments. Here, we review the principal advances and applications performed using this platform during the last years.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.