Genetic Characterization of the Tick-Borne Orbiviruses

Viruses

Manjunatha N. Belaganahalli⁴  Sushila Maan¹  Narender S. Maan¹  Joe Brownlie³  Robert Tesh²  Houssam Attoui⁴  Peter P. C. Mertens⁴ 

[1] Department of Animal Biotechnology, LLR University of Veterinary and Animal Sciences, Hisar 125 004, Haryana, India; E-Mail:;Department of Pathology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555–0609, USA; E-Mail:;Department of Pathology and Infectious Diseases, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts AL9 7TA, UK; E-Mail:;Vector-borne Viral Diseases Programme, The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK; E-Mails:

关键词: Chenuda virus;    Chobar Gorge virus;    Wad Medani virus;    Great Island virus;    Kemorovo virus;    Orbivirus;    Reoviridae;    full genome;    dsRNA virus;    sequencing;   

DOI  :  10.3390/v7052185

来源: mdpi

【 摘 要 】

The International Committee for Taxonomy of Viruses (ICTV) recognizes four species of tick-borne orbiviruses (TBOs): Chenuda virus, Chobar Gorge virus, Wad Medani virus and Great Island virus (genus Orbivirus, family Reoviridae). Nucleotide (nt) and amino acid (aa) sequence comparisons provide a basis for orbivirus detection and classification, however full genome sequence data were only available for the Great Island virus species. We report representative genome-sequences for the three other TBO species (virus isolates: Chenuda virus (CNUV); Chobar Gorge virus (CGV) and Wad Medani virus (WMV)). Phylogenetic comparisons show that TBOs cluster separately from insect-borne orbiviruses (IBOs). CNUV, CGV, WMV and GIV share low level aa/nt identities with other orbiviruses, in ‘conserved’ Pol, T2 and T13 proteins/genes, identifying them as four distinct virus-species. The TBO genome segment encoding cell attachment, outer capsid protein 1 (OC1), is approximately half the size of the equivalent segment from insect-borne orbiviruses, helping to explain why tick-borne orbiviruses have a ~1 kb smaller genome.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.