International Journal of Molecular Sciences | |
Evaluation and Selection of Appropriate Reference Genes for Real-Time Quantitative PCR Analysis of Gene Expression in Nile Tilapia ( |
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Erlong Wang1  Kaiyu Wang1  Defang Chen2  Jun Wang1  Yang He1  Bo Long1  Lei Yang1  Qian Yang1  Yi Geng1  Xiaoli Huang2  Ping Ouyang1  Weimin Lai1  | |
[1] Department of Basic Veterinary, Sichuan Agricultural University, Wenjiang District Huimin Road No. 211, Chengdu 611130, China; E-Mails:;Department of Aquaculture, Sichuan Agricultural University, Wenjiang District Huimin Road No. 211, Chengdu 611130, China; E-Mails: | |
关键词:
real time quantitative PCR;
reference gene;
stability;
normalization;
tilapia;
rSip;
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DOI : 10.3390/ijms16059998 | |
来源: mdpi | |
【 摘 要 】
qPCR as a powerful and attractive methodology has been widely applied to aquaculture researches for gene expression analyses. However, the suitable reference selection is critical for normalizing target genes expression in qPCR. In the present study, six commonly used endogenous controls were selected as candidate reference genes to evaluate and analyze their expression levels, stabilities and normalization to immune-related gene
【 授权许可】
CC BY
© 2015 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
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