Extracts from Annona Muricata L. and Annona Reticulata L. (Annonaceae) Potently and Selectively Inhibit Plasmodium Falciparum

Medicines

Lauve Rachel Tchokouaha Yamthe²  Patrick Valere Tsouh Fokou²  Cedric Derick Jiatsa Mbouna²  Rodrigue Keumoe²  Bruno Lenta Ndjakou³  Paul Toukam Djouonzo¹  Alvine Ngoutane Mfopa²  Jennifer Legac⁴  Nole Tsabang¹  Jiri Gut⁴  Philip J. Rosenthal⁴  Fabrice Fekam Boyom² 

[1] Institute of Medical Research and Medicinal Plants Studies (IMPM), P.O. Box 6163, Yaoundé, Cameroon; E-Mails:;Laboratory for Phytobiochemistry and Medicinal Plants Studies, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon; E-Mails:;Department of Chemistry, Higher Teacher Training College, University of Yaoundé 1. P.O. Box 47, Yaoundé, Cameroon; E-Mail:;Division of Infectious Diseases, Department of Medicine, University of California, San Francisco, CA 94943, USA; E-Mails:

关键词: Annona muricata;    Annona reticulata;    cytotoxicity;    Plasmodium falciparum;    antiplasmodial activity;   

DOI  :  10.3390/medicines2020055

来源: mdpi

【 摘 要 】

The aim of this work was to screen extracts from Annona muricata and Annona reticulata in vitro against Plasmodium falciparum. Crude ethanolic extracts, methylene chloride fractions, aqueous fractions, subfractions and isolated compounds (stigmasterol-3-O-β-d-glucopyranoside, lichexanthone, gallic acid and β-sitosterol-3-O-β-d-glucopyranoside) were tested for cytotoxicity on erythrocytes and Human Foreskin Fibroblasts cells and against the W2 strain of P. falciparum in culture. Results indicated that none of the extracts was cytotoxic at concentrations up to 10 µg/mL. Most of the extracts, fractions and subfractions inhibited the growth of P. falciparum with IC₅₀ values ranging from 0.07 to 3.46 µg/mL. The most potent was the subfraction 30 from A. muricata stem bark (IC₅₀ = 0.07 µg/mL) with a selectivity index of ˃ 142. Subfraction 3 from A. muricata root also exhibited very good activity (IC₅₀ = 0.09 µg/mL) with a high selectivity index (SI ˃ 111). Amongst the isolated compounds, only gallic acid showed activity with IC₅₀ of 3.32 µg/mL and SI > 10. These results support traditional claims for A. muricata and A. reticulata in the treatment of malaria. Given their limited cytotoxicity profile, their extracts qualify as promising starting points for antimalarial drug discovery.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.