Effect of Chum Salmon Egg Lectin on Tight Junctions in Caco-2 Cell Monolayers

Molecules

Ryo Nemoto²  Shintaro Yamamoto²  Tomohisa Ogawa²  Ryno Naude¹  Koji Muramoto² 

[1] Department of Biochemistry and Microbiology, Nelson Mandel Metropolitan University, Port Elizabeth 6031, South Africa; E-Mail:;Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai 980-8577, Japan; E-Mails:

关键词: Caco-2 cell;    intestinal transport;    lectin;    tight junction;    transepithelial transport;   

DOI  :  10.3390/molecules20058094

来源: mdpi

【 摘 要 】

The effect of a chum salmon egg lectin (CSL3) on tight junction (TJ) of Caco-2 cell monolayers was investigated. The lectin opened TJ as indicated by the decrease of the transepithelial electrical resistance (TER) value and the increase of the permeation of lucifer yellow, which is transported via the TJ-mediated paracellular pathway. The effects of CSL3 were inhibited by the addition of 10 mM L-rhamnose or D-galactose which were specific sugars for CSL3. The lectin increased the intracellular Ca²⁺ of Caco-2 cell monolayers, that could be inhibited by the addition of L-rhamnose. The fluorescence immunostaining of β-actin in Caco-2 cell monolayers revealed that the cytoskeleton was changed by the CSL3 treatment, suggesting that CSL3 depolymerized β-actin to cause reversible TJ structural and functional disruption. Although Japanese jack bean lectin and wheat germ lectin showed similar effects in the decrease of the TER values and the increase of the intracellular Ca²⁺, they could not be inhibited by the same concentrations of simple sugars, such as D-glucose and N-acetyl-D-glucosamine.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.