Qualitative and Quantitative Analysis of Lignan Constituents in Caulis Trachelospermi by HPLC-QTOF-MS and HPLC-UV

Molecules

Xiao-Ting Liu¹  Xu-Guang Wang¹  Yu Yang¹  Rui Xu¹  Fan-Hua Meng¹  Neng-Jiang Yu¹  Yi-Min Zhao¹ 

[1] Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China; E-Mails:

关键词: Caulis Trachelospermi;    HPLC-QTOF-MS;    HPLC-UV;    dibenzylbutyrolatone lignans;   

DOI  :  10.3390/molecules20058107

来源: mdpi

【 摘 要 】

A high-performance liquid chromatography coupled with quadrupole tandem time-of-flight mass (HPLC-QTOF-MS) and ultraviolet spectrometry (HPLC-UV) was established for simultaneous qualitative and quantitative analysis of the major chemical constituents in Caulis Trachelospermi, respectively. The analysis was performed on an Agilent Zorbax Eclipse Plus C18 column (4.6 mm × 150 mm, 5 μm) using a binary gradient system of water and methanol, with ultraviolet absorption at 230 nm. Based on high-resolution ESI-MS/MS fragmentation behaviors of the reference standards, the characteristic cleavage patterns of lignano-9, 9'-lactones and lignano-8'-hydroxy-9, 9'-lactones were obtained. The results demonstrated that the characteristic fragmentation patterns are valuable for identifying and differentiating lignano-9,9'-lactones and lignano-8'-hydroxy-9,9'-lactones. As such, a total of 25 compounds in Caulis Trachelospermi were unambiguously or tentatively identified via comparisons with reference standards or literature. In addition, 14 dibenzylbutyrolatone lignans were simultaneously quantified in Caulis Trachelospermi by HPLC-UV method. The method is suitable for the qualitative and quantitative analyses of dibenzylbutyrolatone lignans in Caulis Trachelospermi.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.