Impact of Leucine Supplementation on Exercise Training Induced Anti-Cardiac Remodeling Effect in Heart Failure Mice

Nutrients

Wilson Max Almeida Monteiro de Moraes²  Thaís Plasti Melara¹  Pamella Ramona Moraes de Souza³  Fabiana de Salvi Guimar฾s¹  Luiz Henrique Marchesi Bozi¹  Patricia Chakur Brum¹ 

[1] School of Physical Education and Sport, University of São Paulo, São Paulo, 05508-900, Brazil; E-Mails:;Biosciences Department, Federal University of São Paulo, Santos, 11015-020, Brazil; E-Mail:;Post-graduation Program in Medicine, Nove de Julho University (UNINOVE), São Paulo, 01504-001, Brazil; E-Mail:

关键词: heart failure;    aerobic exercise training;    leucine;    cardiac remodeling;   

DOI  :  10.3390/nu7053751

来源: mdpi

【 摘 要 】

Leucine supplementation potentiates the effects of aerobic exercise training (AET) on skeletal muscle; however, its potential effects associated with AET on cardiac muscle have not been clarified yet. We tested whether leucine supplementation would potentiate the anti-cardiac remodeling effect of AET in a genetic model of sympathetic hyperactivity-induced heart failure in mice (α_2A/α_2CARKO). Mice were assigned to five groups: wild type mice treated with placebo and sedentary (WT, n = 11), α_2A/α_2CARKO treated with placebo and sedentary (KO, n = 9), α_2A/α_2CARKO treated with leucine and sedentary (KOL, n = 11), α_2A/α_2CARKO treated with placebo and AET (KOT, n = 12) or α_2A/α_2CARKO treated with leucine and AET (KOLT, n = 12). AET consisted of four weeks on a treadmill with 60 min sessions (six days/week, 60% of maximal speed) and administration by gavage of leucine (1.35 g/kg/day) or placebo (distilled water). The AET significantly improved exercise capacity, fractional shortening and re-established cardiomyocytes’ diameter and collagen fraction in KOT. Additionally, AET significantly prevented the proteasome hyperactivity, increased misfolded proteins and HSP27 expression. Isolated leucine supplementation displayed no effect on cardiac function and structure (KOL), however, when associated with AET (KOLT), it increased exercise tolerance to a higher degree than isolated AET (KOT) despite no additional effects on AET induced anti-cardiac remodeling. Our results provide evidence for the modest impact of leucine supplementation on cardiac structure and function in exercised heart failure mice. Leucine supplementation potentiated AET effects on exercise tolerance, which might be related to its recognized impact on skeletal muscle.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.