Effect of COD:SO42− Ratio, HRT and Linoleic Acid Concentration on Mesophilic Sulfate Reduction: Reactor Performance and Microbial Population Dynamics

Water

Chungman Moon¹  Rajesh Singh¹  Sathyanarayan S. Veeravalli¹  Saravanan R. Shanmugam¹  Subba Rao Chaganti²  Jerald A. Lalman¹  Daniel D. Heath² 

[1] Department of Civil and Environmental Engineering, University of Windsor, 401 Sunset Ave., Windsor, ON N9B 3P4, Canada; E-Mails:;Great Lakes Institute for Environmental Research, University of Windsor, 401 Sunset Ave., Windsor, ON N9B 3P4, Canada; E-Mails:

关键词: sulfate reduction;    sulfate reducing bacteria;    methanogens;    anaerobic sequencing batch reactor;    COD/SO₄²⁻ ratio;   

DOI  :  10.3390/w7052275

来源: mdpi

【 摘 要 】

Biological sulfate (SO₄²⁻) reduction was examined in anaerobic sequential batch reactors (ASBRs) operated under different hydraulic retention times (HRTs) ranging from 12 to 36 h and COD (Chemical Oxygen Demand)/SO₄²⁻ ratios of 2.4, 1.6 and 0.8. Competition between SO₄²⁻ reducing bacteria (SRBs), methane producing archaea (MPAs) and homoacetogens (HACs) was examined in controls and cultures treated with linoleic acid (LA). The ASBR performance was influenced by the COD/SO₄²⁻ ratio in control cultures with a SO₄²⁻ reduction of 87% at a COD/SO₄²⁻ ratio of 0.8. At a 12 h HRT, in both control and LA treated cultures, greater than 75% SO₄²⁻ removal was observed under all the conditions examined. In control reactors operating at a 36 h HRT, high levels of MPAs belonging to Methanobacteriales and Methanosarcinales were detected; however, in comparison, under low COD/SO₄²⁻ ratio and with decreasing HRT conditions, a relative increase in SRBs belonging to Desulfovibrio and Desulfatibacillum was observed. Adding 0.5 g·L⁻¹ LA suppressed Methanobacteriales, while increasing the LA concentration to 1 g·L⁻¹ completely suppressed MPAs with a relative increase in SRBs. HACs belonging to Bacteroidetes were observed in the control and in cultures operated at 12 h HRT with a COD/SO₄²⁻ ratio of 1.6 and fed 0.5 g·L⁻¹ LA; however, with all other LA levels (0.5 and 1.0 g·L⁻¹) and HRTs (12, 24 and 36 h), HACs were not detected.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.