MicroRNA-27a-3p Inhibits Melanogenesis in Mouse Skin Melanocytes by Targeting Wnt3a

International Journal of Molecular Sciences

Yuanyuan Zhao¹  Pengchao Wang¹  Jinzhu Meng¹  Yuankai Ji¹  Dongmei Xu¹  Tianzhi Chen¹  Ruiwen Fan¹  Xiuju Yu¹  Jianbo Yao¹  Changsheng Dong¹ 

[1] College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China; E-Mails:

关键词: melanogenesis;    Wnt3a;    miR-27a-3p;    post-transcriptional regulation;   

DOI  :  10.3390/ijms160510921

来源: mdpi

【 摘 要 】

MicroRNAs (miRNAs) play an essential role in the regulation of almost all the biological processes, including melanogenesis. MiR-27a-3p is nearly six times higher in white alpaca skin compared to brown skin, which indicates that miR-27a-3p may be a candidate regulator for melanogenesis. Wnt3a plays an important role in promoting melanoblasts to differentiate into melanocytes and melanogenesis. To confirm the function of miR-27a-3p to melanogenesis in mammals, miR-27a-3p mimic, inhibitor and their negative control were transfected into mouse melanocytes. As a result, miR-27a-3p inhibits melanogenesis by repressing Wnt3a at post-transcriptional level. A significant decrease in Wnt3a luciferase activity was observed in 293T cells co-transfected with the matched luciferase reporter vector and pre-miR-27a. Furthermore, the presence of exogenous miR-27a-3p significantly decreased Wnt3a protein expression rather than mRNA and reduced β-catenin mRNA levels in melanocytes. The over-expression of miR-27a-3p significantly increased the melanin content of melanocytes. However, miR-27a-3p inhibitor performs an opposite effect on melanogenesis. Wnt3a is one target of miR-27a-3p. MiR-27a-3p could inhibit Wnt3a protein amount by post-transcriptional regulation and melanogenesis in mouse melanocytes. Previous studies reported that Wnt3a promoted melanogenensis in mouse melanocytes. Thus, miR-27-3p inhibits melanogenesis by repressing Wnt3a protein expression.

【 授权许可】

CC BY   
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