Simple and Rapid Determination of Ethanol Content in Beer Using an Amperometric Biosensor

Chemosensors

Vojtᆼh Polan¹  Aleš Eisner¹  Karel Vytᖚs¹  Paolo Ugo² 

[1] Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 53210 Pardubice, Czech Republic;

关键词: ethanol;    amperometry;    screen-printed carbon electrode;    alcohol dehydrogenase;    biosensor;    beer;   

DOI  :  10.3390/chemosensors3020169

来源: mdpi

【 摘 要 】

An alcohol dehydrogenase-based biosensor was prepared and tested for its use to determine ethanol in beer. The biosensor is based on a screen-printed carbon electrode (SPCE) modified by rhodium dioxide and immobilized with a biocatalytic layer containing the enzyme. Function of the enzyme biosensor was tested in model ethanol samples, in which it showed a linear range of 15–120 g∙L⁻¹ with a detection limit of 3.3 g∙L⁻¹ (established as 3σ) and response time of 19 s. In a potential window from –0.2 to +0.45 V, interferences of both ascorbic and uric acids were negligible. Several types of marketed beers of Czech provenance were selected and subjected to measurements under optimized conditions but without any pretreatment of real samples. When compared with the reference method (gas chromatography), the results were in quite good agreement for beers of the pale lager type but higher contents of ethanol were indicated in the samples of dark lager beers.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.