Computational Approach to 3D Modeling of the Lymph Node Geometry

Computation

Alexey Kislitsyn¹  Rostislav Savinkov¹  Mario Novkovic³  Lucas Onder³  Gennady Bocharov⁴  Filippo Castiglione² 

[1] Lomonosov Moscow State University, Lenenskie Gori 1, Moscow 119991, Russia; E-Mails:Lomonosov Moscow State University, Lenenskie Gori 1, Moscow 119991, Russia;;Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen 9007, Switzerland; E-Mails:;Institute of Numerical Mathematics, Russian Academy of Sciences, Gubkina 8, Moscow 119333, Russia

关键词: lymph node;    FRC network;    geometric model;    computational method;   

DOI  :  10.3390/computation3020222

来源: mdpi

【 摘 要 】

In this study we present a computational approach to the generation of the major geometric structures of an idealized murine lymph node (LN). In this generation, we consider the major compartments such as the subcapsular sinus, B cell follicles, trabecular and medullar sinuses, blood vessels and the T cell zone with a primary focus on the fibroblastic reticular cell (FRC) network. Confocal microscopy data of LN macroscopic structures and structural properties of the FRC network have been generated and utilized in the present model. The methodology sets a library of modules that can be used to assemble a solid geometric LN model and subsequently generate an adaptive mesh model capable of implementing transport phenomena. Overall, based on the use of high-resolution confocal microscopy and morphological analysis of cell 3D reconstructions, we have developed a computational model of the LN geometry, suitable for further investigation in studies of fluid transport and cell migration in this immunologically essential organ.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.